| 1. | After dialysis , the recombinant gst - ri was purified by rnase a - sepharose 4b affinity chromatography , and the single band of gst - ri was showed on a sds - electrophoresis gel to remove the extra urea
复性的gst一班经knasea - sepharose4b亲合层析纯化， sds一page鉴定得到单一的gst一rj条带。 |
| 2. | After the lysate was purified with gst sepharose 4b affinity chromatography , a specific band of 34kd appeared in sds - page gel , the purity and the titer of the antibody against fusion protein gst - hnadc3 could reach up to 90 % and 1 : 32 , respectively
菌体裂解液经gst亲和层析纯化后， sds page上出现一分子量为34kd的特异蛋白条带，纯度可达90 。 |
| 3. | In order to attain bioactive glycoprotein , glycoprotein of the leaves of camellia sinensis , was purified from coarse glycoproteins purified by sephadex g - 100 gel chromatography , by cona - sepharose 4b affinity chromatography
为了纯化天然糖蛋白，进行了茶树叶糖蛋白的cona - sepharose4b亲和层析，从sephadexg - 100凝胶过滤收集的粗糖蛋白中，分离茶树叶糖蛋白。 |
| 4. | Then the product was purified by glutathione sepharose 4b chelation affinity chromatography , and upper purified gst - gnrh / trs fusion proteins was received for further the foundations established in the scientific research and the real application
表达产物经谷胱甘肽琼脂糖4b亲和层析纯化后，得到了纯度较高的gst - gnrh trs融合蛋白。为进一步科研和实际应用奠定基础。 |
| 5. | We constructed the pgex - gdnf expression vecto ' rs and obtained the recombinant protein of expected size after induction of iptg . the fusion protein was purified using glutathione sepharose 4b affinity chromatography
将大熊猫和朱?的gdnf基因片段分别克隆至pgex ? 4t ? 3表达载体，并经iptg诱导，表达出了预期大小的蛋白，该蛋白用glutathionesepharose4bmicrospincolumn进行了纯化。 |
| 6. | Purified fusion protein gst - hnadc3 was used as an immunogen to inoculate rabbits and the antibody against the gst - hnadc3 fusion protein was raised , and was purified by gst sepharose 4b affinity chromatography to remove the antibody against gst
Ptg诱导表达，超声破碎细胞后，采用亲和层析方法纯化融合蛋白gst十nadc3 ，并以此为抗原免疫新西兰株白兔制备融合蛋白抗体。应用亲和层析的方法对gst十nadc3融合蛋白抗体进行纯化，以去除抗gst抗体。 |
| 7. | In this study , a novel strategy to inhibit the activation of the complement system has been developed . to get the purified clq as the target molecule for biopanning , the first step of our purification was to isolate the clq from clr2cls2 by affinity chromatography on igg - sepharose 4b column
本研究另辟蹊径，以补体经典激活途径始动分子c1q为靶标，采用噬菌体肽库技术，亲和筛选能与c1q结合的噬菌体克隆，研制可抑制补体激活的c1q模拟短肽。 |
| 8. | Crotalaria mucronata lectin ( cml ) was purified from seeds of crotalaria mucronata by extraction , fraction with ( nhi ) 2864 , hog gastric mucin - sepharose 4b affinity chromatography and followed by gel filtration of sephacryl s - 200 hr . cml agglutinated type a human red blood cells specially . the purified cml gave one band pel e ' ectronhoresis and on sds nolvacrvlamide gel electrophoresis
野花生豆( crotalariamucronata )经磨粉、浸取、硫酸铵分级沉淀、猪胃粘蛋白- sepharose4b亲和层析、 sephacryls - 200hr分子筛层析可得到一表观分子量为103kd且对a型血红细胞专一凝集的野花生豆凝集素( crotalariamucronatalectin ， cml ) 。 |
| 9. | One is a combination of ion exchange chromatography on q sepharose ff , hydrophobic interaction chromatography on phenyl hp , gel filtration chromatography on sephadex 200 and higher resolution ion exchange chromatography on mono q . the other is a combination of ion exchange chromatography on q sepharose ff , two affinity chromatography on con a sepharose 4b and benzamidine sepharose 6bcl sequentially
上清液中重组蝗蛇毒类激血酶的纯化是通过设计的不同分离纯化方法组合进行的，并对每一种组合进行了活力与纯度及口收率的评价，结果表明两种组合方式都具有一定的实用性和可操作性。 |
| 10. | After purification using glutathione sepharose 4b affinity chromatography and digestion with thrombin , the recombinant ntfs were found to be biologically active in the pc 12 cells neurite outgrowth assay . the assays demonstrated that the purified ntfs proteins exhibited normal activity , which is the first step in developing a comprehensive gene therapy for nerve diseases of the giant panda and the crested ibis
最后，对重组表达蛋白经glutathlonesepharose4bmicrospincolumn亲和纯化后，进行大鼠肾上腺嗜铬瘤细胞（ p2 ）神经营养因子的活性鉴定，发现其能够诱导神经细胞分化产生突触，即具有预期的生物学活性，表明所获表达蛋白为大熊猫nt |
